Recombinant Human soluble RANK Ligand

Molecule:

RANKL

Synonyms:

Tumor necrosis factor ligand superfamily member 11, Receptor activator of nuclear factor kappa-B ligand, RANKL, CD254, Osteoclast differentiation factor, ODF, TNF-related activation-induced cytokine, TRANCE

Species:

Human

Cat. No.:

CRR100B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$185.00 BUY

Description:

Receptor Activator of NF-κB Ligand (RANKL) is a cell-bound marker (CD254) related to the TNF family of proteins. RANKL plays a critical role in bone metabolism, particularly osteoclast differentiation. In addition, RANKL is expressed by some T cells is thought to promote dendritic cell maturation.

Entrez Symbol:

TNFSF11

Uniprot Name:

Tumor necrosis factor ligand superfamily member 11

Uniprot ID:

O14788

Data PDF:

CRR100

Source:

E. coli

Molecular Weight:

19.7 kDa (175 aa)

Physical State:

Lyophilized

Formulation:

Sterile filtered aqueous solution containing 10 mM Na2PO4, pH 7.5

Purity:

≥ 95.0% as determined by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 ≤ 50 ng/mL determined by activity of RAW-BlueTM cells. This product demonstrates dose-dependent stimulation of IL-8 production in human PBMC.

Specific Activity:

≥ 2.0 x 104 units/mg

Endotoxin Level:

< 1 EU/μg protein, by kinetic LAL analysis.

Amino Acid Sequence:

EKAMVDGSWL DLAKRSKLEA QPFAHLTINA TDIPSGSHKV SLSSWYHDRG WAKISNMTFS NGKLIVNQDG FYYLYANICF RHHETSGDLA TEYLQLMVYV TKTSIKIPSS HTLMKGGSTK YWSGNSEFHF YSINVGGFFK LRSGEEISIE VSNPSLLDPD QDATYFGAFK VRDID

Reconstitution:

Centrifuge vial prior to opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/ml. DO NOT VORTEX.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.