Recombinant Human TWEAK Receptor

Molecule:

TWEAKR

Synonyms:

CD266, FN14, TWEAKR

Species:

Human

Cat. No.:

CRT601B pdf (datasheet)

Quantity/Size:

25 μg

Price:

$235.00 BUY

Description:

Human TNF-related weak inducer of apoptosis receptor (TWEAKR) also known as Tumor necrosis factor receptor superfamily member 12A precursor (gene name TNFRSF12A) or fibroblast growth factor-inducable 14 kD protein, is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. It is expressed in the spleen, thymus, peripheral blood lymphocytes, colon, and small intestine. Signal transduction by TWEAKR can be activated by either the membrane anchored or the soluble TWEAK. In addition, It plays a role in TWEAKinduced endothelial cell migration, proliferation, and angiogenesis. Recombinant Human TWEAK Receptor is a single non-glycosylated polypeptide chain containing 53 amino acids.

Entrez Symbol:

TNFRSF12A

Uniprot Name:

Tumor necrosis factor receptor superfamily member 12A

Uniprot ID:

Q9NP84

Data PDF:

CRT601

Source:

E. coli

Molecular Weight:

5.6 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile filtered solution in PBS, pH 7.4.

Purity:

>95% by HPLC and SDS-PAGE

Biological Activity:

Fully biologically active when compared to the standard. The ED50 is determined by inhibiting TWEAK dependent proliferation of human umbilical vein endothelial cells (HUVEC) in the presence of 15 ng/mL of recombinant human TWEAK and is less than 30 ng/mL.

Specific Activity:

>3.3 × 10e4 IU/mg

Endotoxin Level:

<0.1 ng/οΩΫg of rHuTWEAKR as determined by LAL method

Amino Acid Sequence:

EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LWP

Reconstitution:

Centrifuge vial prior to opening. Add sterile water or aqueous buffer containing 0.1% BSA to the vial to a concentration 0.1-1.0 mg/mL to fully solubilize the protein.

Storage/Stability:

Store as supplied at -20 °C for up to one year. Upon reconstitution under sterile conditions, store at 2-8 °C for one month, or in aliquots at -20 °C to -80 °C for up to 3 months. Avoid repeated freeze-thaw cycles.