Recombinant Human TWEAK Receptor

Molecule:

TWEAKR

Synonyms:

CD266, FN14, TWEAKR

Species:

Human

Cat. No.:

CRT601B pdf (datasheet)

Quantity/Size:

25 μg

Price:

$275.00 BUY

Description:

Human TNF-related weak inducer of apoptosis receptor (TWEAKR) also known as Tumor necrosis factor receptor superfamily member 12A precursor (gene name TNFRSF12A) or fibroblast growth factor-inducable 14 kD protein, is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. It is expressed in the spleen, thymus, peripheral blood lymphocytes, colon, and small intestine. Signal transduction by TWEAKR can be activated by either the membrane anchored or the soluble TWEAK. In addition, It plays a role in TWEAKinduced endothelial cell migration, proliferation, and angiogenesis.

Entrez Symbol:

TNFRSF12A

Uniprot Name:

Tumor necrosis factor receptor superfamily member 12A

Uniprot ID:

Q9NP84

Data PDF:

CRT601

Source:

E. coli

Molecular Weight:

5.6 kDa (53 aa)

Formulation:

Lyophilized from a sterile filtered solution in PBS, pH 7.4.

Purity:

>95% by HPLC and SDS-PAGE analyses

Biological Activity:

ED50 < 30 ng/ml, determined by inhibiting TWEAK-dependent proliferation of human umbilical vein endothelial cells (HUVEC) HUVEC cells in the presence of 15 ng/ml rhTWEAK

Specific Activity:

> 3.3 × 104 IU/mg

Endotoxin Level:

<1 EU/µg by LAL analysis

Amino Acid Sequence:

EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LWP

Reconstitution:

Centrifuge vial prior to opening. Add sterile water or aqueous buffer containing 0.1% BSA to the vial to a concentration 0.1-1.0 mg/mL to fully solubilize the protein.

Storage/Stability:

Store as supplied at -20°C for up to one year. Upon reconstitution under sterile conditions, store at 2-8 °C for one month, or in aliquots at -20°C to -80°C for up to 3 months. Avoid repeated freeze-thaw cycles.