Recombinant Human VEGF 165

Molecule:

VEGFA

Synonyms:

Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF

Species:

Human

Cat. No.:

CRV000B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$185.00 BUY

Description:

Vascular Endothelial Growth Factor is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells. VEGF-A is also a vasodilator and increases microvascular permeability and was originally referred to as vascular permeability factor. There are multiple isoforms of VEGF-A that result from alternative splicing of mRNA from a single, 8-exon VEGFA gene, with VEGF-165 being the most abundant. The VEGF-165 isoform is a secreted protein that acts on receptors VEGFR-1 and VEGFR-2 to modulate endothelial cell proliferation and angiogenesis.

Entrez Symbol:

VEGFA

Uniprot Name:

Vascular endothelial growth factor A

Uniprot ID:

P15692-4

Data PDF:

CRV000

Source:

E. coli

Molecular Weight:

19.3/38.6 kDa (166/332 aa), dimer

Physical State:

Lyophilized

Formulation:

Sterile filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA).

Purity:

> 95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 < 10 ng/ml, determined by dose-dependent cell proliferation assay using human umbilical vein endothelial cells (HUVEC).

Specific Activity:

1.0 × 105 units/mg

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis

Amino Acid Sequence:

APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. Suspend the product by gently pipetting the solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Store at -20°C to -80°C for up to 1 year.