Recombinant Human VEGF 189

Molecule:

VEGF

Synonyms:

VEGFA, Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, MVCD1

Species:

Human

Cat. No.:

CRV114A pdf (datasheet)

Quantity/Size:

2 μg

Price:

$235.00 BUY

Description:

VEGF is a member of the platelet-derived growth factor family. It is a specific mitogen for vascular endothelial cells and a strong angiogenic factor in vivo. Five different proteins are generated by diffential splicing: VEGF121, VEGF145, VEGF165, VEGF189 and VEGF206. The most abdundant form is VEGF165. Whereas VEGF121 and VEGF165 are secreted proteins, VEGF145, VEGF189 and VEGF206 are strongly cell-associated. The isoforms VEGF145, VEGF165 and VEGF189 bind to heparin with high affinity. VEGF165 is apparently a homodimer, but preparations of VEGF165 show some heterogeneity on SDS gels, depending on the secretion of different glycosylation patterns. All dimeric forms have similar biological activities but their bio-availability is very different. There is good evidence that heterodimeric molecules between the different isoforms also exist and that different cells and tissues express different VEGF isoforms. The other members of this increasing growth factor family are VEGF-B, -C, -D and -E. Another member is the Placenta growth factor PlGF.

Entrez Symbol:

VEGFA

Uniprot Name:

vascular endothelial growth factor

Uniprot ID:

P15692-2

Data PDF:

CRV114

Source:

E. coli

Molecular Weight:

~42 kDa

Formulation:

Lyophilized from a sterile filtered solution in 50 mM Acetic acid.

Purity:

>98% by SDS-PAGE analysis.

Biological Activity:

ED50 typically 2-10 ng/ml, determined by a cell proliferation assay using primary human umbilical vein endonthelial cells (HUVEC) and primary human dermal lymphatic endothelial cells (HDLEC).

Endotoxin Level:

< 1 EU/μg

Amino Acid Sequence:

APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQEKKSVRG KGKGQKRKRK KSRYKSWSVP CGPCSERRKH LFVQDPQTCK CSCKNTDSRC KARQLELNER TCRCDKPRR

Storage/Stability:

Stable for 1 year at -20°C to -80°C