Recombinant Human soluble VEGFR-1 D1-5

Molecule:

VEGFR1/FLT1

Synonyms:

Vascular endothelial growth factor receptor 1, fms-like tyrosine kinase 1, FLT-1

Species:

Human

Cat. No.:

CRF102A pdf (datasheet)

Quantity/Size:

5 μg

Price:

$220.00 BUY

Description:

Recombinant human soluble Vascular Endothelial Growth Factor Receptor-1 domain D1-5 (sVEGFR-1D1-5) is produced as a non-chimeric protein in a monomeric form. The soluble receptor protein contains only the first 5 extracellular domains, which contain all the information necessary for binding of VEGF.

Endothelial cells express three different vascular endothelial growth factor (VEGF) receptors, belonging to the family of receptor tyrosine kinases (RTKs). They are named VEGFR-1 (Flt-1), VEGFR-2 (KDR/Flk-1), VEGFR-3 (Flt-4). Their expression is almost exclusively restricted to endothelial cells, but VEGFR-1 can also be found on monocytes, dendritic cells and on trophoblast cells. The flt-1 gene was first described in 1990. The receptor contains seven immunoglobulin-like extracellular domains, a single transmembrane region and an intracellular splited tyrosine kinase domain. Compared to VEGFR-2 the Flt-1 receptor has a higher affinity for VEGF but a weaker signaling activity. VEGFR-1 thus leads not to proliferation of endothelial cells, but mediates signals for differentiation. Interestingly a naturally occuring soluble variant of VEGFR-1 (sVEGFR-1) was found in HUVE supernatants in 1996, which is generated by alternative splicing of the flt-1 mRNA. The biological functions of sVEGFR-1 still are not clear, but it seems to be an endogenous regulator of angiogenesis, binding VEGF with the same affinity as the full-length receptor.

Entrez Symbol:

FLT1

Uniprot Name:

Vascular endothelial growth factor receptor 1

Uniprot ID:

P17948

Data PDF:

CRF102

Source:

Insect cells

Molecular Weight:

70 kDa (536 aa) monomer

Formulation:

Lyophilized from a sterile filtered solution in PBS.

Purity:

> 90% as determined by SDS PAGE and visualized by silver stain

Biological Activity:

The activity of sVEGFR-1 D1-5 was determined by its ability to inhibit the VEGF-Ainduced proliferation of HUVECs.

Endotoxin Level:

< 1 EU/mg

Amino Acid Sequence:

SKLKDPELSL KGTQHIMQAG QTLHLQCRGE AAHKWSLPEM VSKESERLSI TKSACGRNGK QFCSTLTLNT AQANHTGFYS CKYLAVPTSK KKETESAIYI FISDTGRPFV EMYSEIPEII HMTEGRELVI PCRVTSPNIT VTLKKFPLDT LIPDGKRIIW DSRKGFIISN ATYKEIGLLT CEATVNGHLY KTNYLTHRQT NTIIDVQIST PRPVKLLRGH TLVLNCTATT PLNTRVQMTW SYPDEKNKRA SVRRRIDQSN SHANIFYSVL TIDKMQNKDK GLYTCRVRSG PSFKSVNTSV HIYDKAFITV KHRKQQVLET VAGKRSYRLS MKVKAFPSPE VVWLKDGLPA TEKSARYLTR GYSLIIKDVT EEDAGNYTIL LSIKQSNVFK NLTATLIVNV KPQIYEKAVS SFPDPALYPL GSRQILTCTA YGIPQPTIKW FWHPCNHNHS EARCDFCSNN EESFILDADS NMGNRIESIT QRMAIIEGKN KMASTLVVAD SRISGIYICI ASNKVGTVGR NISFYITDVP NGFHVN

Reconstitution:

Centrifuge vial prior to opening. Soluble in water and most aqueous buffers. Add deionized water to the vial to fully solubilize the protein to a concentration > 100 ng/ml.

Storage/Stability:

Lyophilized protein is stable for 6 months at -20°C to -80°C. Store reconstituted protein in working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.