Synonyms:
Human WNT-1, WNT-1, WNT1, WNT 1, h-WNT-1, rh-WNT-1, recombinant human WNT -1, recombinant WNT-1, WNT
Description:
WNT1 is a secreted protein that signals through the Frizzled family of cell surface
receptors and is required for normal embryonic development. WNT1 activation induces a
complex signaling cascade that ultimately leads to the increased expression of over fifty
genes. An important component of WNT1 signaling is the stabilization, and resulting
accumulation, of the intracellular signaling protein, beta-catenine. Wnt signaling induces
and maintains the transformed phenotype and, in certain embryonic cell lines, supports
self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins
are associated with tumorigenesis and are present in numerous human breast cancers.
Mature human WNT1 is a glycosylated protein containing 343 amino acid residues.
Recombinant human WNT1 is a non-glycosylated protein containing 343 amino acid
residues.
Uniprot Name:
Proto-oncogene Wnt-1
Molecular Weight:
38.4 kDa
Physical State:
Lyophilized
Formulation:
Lyophilized from a sterile filtered solution without additives
Purity:
>98% by SDS-PAGE and HPLC
Biological Activity:
The ED50 was determined by its ability to enhance BMP2 induced alkaline phosphatase production by mouse ATDC5 cells. The expected ED50 for this effect is 1.5-2.5 ng/mL in the presence of 200 ng/mL of human BMP2.
Endotoxin Level:
< 0.1 ng/µg of WNT1
Reconstitution:
Centrifuge vial prior to opening. First add sterile distilled water to the vial to fully solubilize the protein to a concentration of 0.1-1.0 mg/ml. After complete solubilization of the protein, it can be further diluted to other aqueous solutions.
Storage/Stability:
Store lyophilized protein at -20°C. After reconstitution the protein is stable for 1 week at 4°C or for longer term in working aliquots at -20°C to -80°C. Avoid repeated freeze thaw cycles.
