Recombinant Mouse CD40 Ligand

Synonyms:

TNFSF5, TRAP, CD154, gp39, T-BAM

Species:

Mouse

Cat. No.:

CRC803C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$3,200.00 / Special Price: $2,100.00 BUY

Description:

CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2. Recombinant mouse CD40 Ligand is a non-glycosylated protein, containing the 149 C-terminal amino acids.

Entrez Symbol:

Cd40lg

Uniprot ID:

P27548

Source:

E. coli

Molecular Weight:

16.4 kDa

Physical State:

Lyophilized

Formulation:

10 mM Na2PO4, 0.1 M Arginine, pH 7.5.

Purity:

> 95%, determined by reducing and non-reducing SDS-PAGE.

Biological Activity:

ED50 < 50 ng/ml, determined by the activity of HEK-BlueTM CD40L cells. Typical ED50 for dose-dependent induction of IL-8 by human PBMCs and is1-10 ng/mL.

Specific Activity:

> 2.0 x 104 units/mg with HEK-BlueTM CD40L cells.

Endotoxin Level:

≤ 1 EU/μg protein, by kinetic LAL

Amino Acid Sequence:

MQRGDEDPQI AAHVVSEANS NAASVLQWAK KGYYTMKSNL VMLENGKQLT VKREGLYYVY TQVTFCSNRE PSSQRPFIVG LWLKPSSGSE RILLKAANTH SSSQLCEQQS VHLGGVFELQ AGASVFVNVT EASQVIHRVG FSSFGLLKL

Reconstitution:

Centrifuge vial prior to opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a co

Storage/Stability:

Store in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.