Recombinant Mouse CD40L

Molecule:

CD40

Synonyms:

Cd40lg, TNF-related activation protein, TRAP, TNF ligand superfamily member 5, TNFSF5, Tcell antigen Gp39

Species:

Mouse

Cat. No.:

CRI229 pdf (datasheet)

Quantity/Size:

25 μg

Price:

$520.00 BUY

Description:

CD40, a member of the TNF receptor family, is a cell surface protein expressed on Bcells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T-cells. Signaling though CD40 plays an important role in the proliferation and differentiation of Bcells, and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40-Ligand is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40LG leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE. The soluble form of CD40LG is an 18 kDa protein comprising the entire TNF homologous region of CD40LG and is generated in vivo by an intracellular proteolytic processing of the full length CD40LG. Recombinant mouse CD40LG is a soluble protein containing 149 amino acid residues comprising the receptor binding TNF-like domain of CD40LG.

Entrez Symbol:

Cd40lg

Uniprot ID:

P27548

Data PDF:

CRI229

Source:

E. coli

Molecular Weight:

16.4 kDa (149 aa)

Formulation:

Lyophilized

Purity:

> 98% by SDS-PAGE analysis

Biological Activity:

ED50 < 0.1 µg/ml, determined by its ability to induce TNF-alpha and MIP1-alpha production by mouse splenocytes.

Endotoxin Level:

< 1 EU/µg

Amino Acid Sequence:

MQRGDEDPQI AAHVVSEANS NAASVLQWAK KGYYTMKSNL VMLENGKQLT VKREGLYYVY TQVTFCSNRE PSSQRPFIVG LWLKPSSGSE RILLKAANTH SSSQLCEQQS VHLGGVFELQ AGASVFVNVT EASQVIHRVG FSSFGLLKL

Storage/Stability:

Stable for 1 year at -20°C to -80°C.