Recombinant Mouse CNTF

Molecule:

CNTF

Species:

Mouse

Cat. No.:

CRC402C pdf (datasheet)

Quantity/Size:

1 mg

Price:

$3,560.00 / Special Price: $2,320.00 BUY

Description:

Mouse Ciliary Neurotrophic Factor (Cntf) is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. Cntf has also been shown to prevent the degeneration of motor axons after axotomy. CNTF is highly conserved across species and exhibits cross-species activity. Recombinant Mouse Cntf is a single non-glycosylated polypeptide chain containing 198 amino acids.

Concentration:

1.4 mg/ml

Entrez Symbol:

Cntf

Uniprot Name:

Ciliary neurotrophic factor

Uniprot ID:

P51642

Source:

E. coli

Molecular Weight:

Monomer, 22.6 kDa (198 aa)

Physical State:

Lyophilized

Formulation:

10 mM sodium phosphate, 150 mM NaCl, 10 mM DTT, 50% Glycerol, pH 7.5.

Purity:

> 95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 ≤ 50 ng/ml, as determined by the dose-dependent proliferation of TF-1 cells.

Specific Activity:

≥ 2.0 x 104 units/ml

Endotoxin Level:

≤ 1 EU/μg protein, by kinetic LAL

Amino Acid Sequence:

MAFAEQSPLT LHRRDLCSRS IWLARKIRSD LTALMESYVK HQGLNKNISL DSVDGVPVAS TDRWSEMTEA ERLQENLQAY RTFQGMLTKL LEDQRVHFTP TEGDFHQAIH TLTLQVSAFA YQLEELMALL EQKVPEKEAD GMPVTIGDGG LFEKKLWGLK VLQELSQWTV RSIHDLRVIS SHHMGISAHE SHYGAKQM

Storage/Stability:

Store in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.