Synonyms:
C-X-C motif chemokine 2, Macrophage inflammatory protein 2, MIP2
Description:
Macrophage Inflammatory Protein-2 (MIP-2) was originally identified as a heparin binding
protein secreted from a mouse macrophage cell line in response to endotoxin stimulation.
Based on its protein and DNA sequences, MIP-2 is a member of the alpha (CXC)
subfamily of chemokines. Similarly to other alpha chemokines, mouse MIP-2 is a potent
chemotactic agent for leukocytes and hematopoietic cells. The expression of MIP-2 was
found to be associated with neutrophil influx in pulmonary inflammation and
glomerulonephritis, suggesting that MIP-2 may contribute to the pathogenesis of
inflammatory diseases.
Uniprot Name:
C-X-C motif chemokine 2
Molecular Weight:
7.9 kDa (73 aa) monomer
Formulation:
Lyophilized from sterile filtered 10 mM sodium phosphate, pH 7.5
Purity:
≥ 95% by reducing and non-reducing SDS-PAGE analysis
Endotoxin Level:
≤ 1 EU/μg as determined by kinetic LAL analysis
Amino Acid Sequence:
AVVASELRCQ CLKTLPRVDF KNIQSLSVTP PGPHCAQTEV IATLKGGQKV CLDPEAPLVQ KIIQKILNKG KAN
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipette the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.
Storage/Stability:
Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.
