Recombinant Mouse GM-CSF Animal Free

Molecule:

CSF2

Synonyms:

GM-CSF, GMCSF, CSF2, Colony stimulating factor 2m MGI1GM

Species:

Mouse

Cat. No.:

CRG101C-AF pdf (datasheet)

Quantity/Size:

1 mg

Price:

$3,100.00 / Special Price: $2,540.00 BUY

Description:

Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. It is produced by endothelial cells, monocytes, fibroblasts and T cells in response to a number of inflammatory mediators.

Manufactured in an Animal-Free facility, without Animal-Derived materials.

Uniprot Name:

Granulocyte-macrophage colony-stimulating factor

Uniprot ID:

P01587

Data PDF:

CRG101-AF

Source:

E. coli

Molecular Weight:

14.3 kDa (125 aa) monomer

Physical State:

Lyophilized

Formulation:

Lyophilized from sterile filtered 10 mM acetic acid.

Purity:

≥ 95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 ≤ 50 pg/ml, determined by the dose-dependent stimulation of the proliferation of mouse FDC-P1 cells.

Specific Activity:

≥ 2.0 x 107 units/mg

Endotoxin Level:

≤ 1.0 EU/µg by kinetic LAL analysis

Amino Acid Sequence:

MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to reconstitute to a recommended concentration of 0.1 mg/mL and gently pipet solution up and down sides of vial. DO NOT VORTEX. Allow several minutes for reconstitution.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.