Recombinant Mouse IFN-gamma

Molecule:

IFN-gamma

Synonyms:

IFN-g, Interferon-gamma, IFN-γ

Species:

Mouse

Cat. No.:

CRI001B pdf (datasheet)

Quantity/Size:

100 μg

Price:

$185.00 BUY

Description:

Interferon gamma (IFN-g) is a soluble cytokine and a type II interferon. IFN-g is secreted by innate and adaptive immune cells. The active protein is a homodimer that binds to the IFN-g receptor, which triggers a cellular response to viral and microbial infections. IFN-g exerts antiproliferative, immunoregulatory and proinflammatory activities and induces the production of cytokines, upregulates the expression of class I and II MHC antigens, Fc receptor and leukocyte adhesion molecules. It modulates macrophage effector functions, influences isotype switching and potentiates the secretion of immunoglobulins by B cells. Additionally, IFN-g augments TH1 cell expansion and may be required for TH1 cell differentiation.

Entrez Symbol:

Ifng

Uniprot Name:

Interferon gamma

Uniprot ID:

P01580

Data PDF:

CRI001

Source:

E. coli

Molecular Weight:

15.7 kDa (134 aa), monomer

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile-filtered aqueous solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5

Purity:

> 95% by reducing and non-reducing SDS-PAGE

Biological Activity:

Viral CPE assay using EMC virus on L929 cells.

Specific Activity:

≥1 x 107 U/mg

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis.

Amino Acid Sequence:

MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.