Synonyms:
IGF1, Somatomedin C, IGF-I, IGFIA, Igf-1
Description:
Background: The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH; MIM 139250). Early
Uniprot Name:
Insulin-like growth factor I
Molecular Weight:
7.600 kDa
Physical State:
Lyophilized
Formulation:
Lyophilized from a sterile filtered solution without additives.
Purity:
>98% by SDS-PAGE and HPLC analyses.
Biological Activity:
ED50: <1.0 ng/ml (Determined by the dose-dependant proliferation of murine BALB\C 3T3 cells, measured by 3H-thymidine uptake).
Endotoxin Level:
<0.1 ng/�g
Amino Acid Sequence:
Gly-Pro-Glu-Thr-Leu (First five N-terminal amino acids)
Reconstitution:
Centrifuge vial prior to opening. Reconstitute in sterile ddH2O to a concentration of ?0.1 mg/ml. The solution can then be further diluted in other aqueous buffers.
Storage/Stability:
Lyophilized protein is stable at room temperature for 3 weeks, but is preferably stored desiccated at -20℃C. After reconstitution, stable for 1 week at 2-4℃C. For long term storage, aliquot and store at -20℃C with a carrier protein (0.1%
