Synonyms:
Somatamedin C, mechano growth factor, IGF-IA
Description:
Insulin-like Growth Factor I, IGF-I, is a growth factor produced in response to growth hormone-stimulated liver and can be found circulating throughout the body and throughout life. IGF-I activates the IGF-I receptor (IGF1R) and the insulin receptor to mediate growth of almost every cell of the body. IGF-I is known as one of the most potent activators of the AKT signaling pathway which is known to be a stimulator of proliferation and an inhibitor of programmed cell death. Mature human IGF-I is 100% homologous with bovine and porcine proteins.
Uniprot Name:
Insulin-like growth factor I
Molecular Weight:
7.7 kDa (70 aa)
Physical State:
Lyophilized
Formulation:
Sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)
Purity:
≥ 95% by reducing and non-reducing SDS-PAGE
Biological Activity:
ED50 < 20 ng/ml, determined by the dose-dependent proliferation of FDC-P1 cells.
Specific Activity:
5.0 x 10e4 U/mg
Endotoxin Level:
≤ 1 EU/μg, by kinetic LAL
Amino Acid Sequence:
GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKAA
Reconstitution:
Centrifuge vial prior to opening. Reconstitute in sterile ddH2O to a concentration of ?0.1 mg/ml. The solution can then be further diluted in other aqueous buffers.
Storage/Stability:
Store as supplied at -20°C to -80°C.
