Recombinant Mouse IL-1 alpha

Molecule:

IL-1 alpha

Synonyms:

IL-1a

Species:

Mouse

Cat. No.:

CRI138A pdf (datasheet)

Quantity/Size:

5 μg

Price:

$85.00 BUY

Description:

Interleukin-1 alpha (IL-1alpha) is a non-secreted proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell-associated cytokine. IL-1α and the independently regulated IL-1β protein have overlapping proinflammatory activities to induce adhesion molecule expression on epithelial cells, control fever induction, initiate rheumatoid arthritis, and promote septic shock.

Uniprot Name:

Interleukin-1 alpha

Uniprot ID:

P01582

Data PDF:

CRI138

Source:

E. coli

Molecular Weight:

18.1 kDa (157 aa) monomer

Formulation:

Lyophilized from sterile filtered 10 mM sodium phosphate, pH 7.5.

Purity:

≥ 95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 < 10 pg/ml as determined by the dose-dependent, induced proliferation of the D10. G4.1 cell line.

Amino Acid Sequence:

MSAPYTYQSD LRYKLMKLVR QKFVMNDSLN QTIYQDVDKH YLSTTWLNDL QQEVKFDMYA YSSGGDDSKY PVTLKISDSQ LFVSAQGEDQ PVLLKELPET PKLITGSETD LIFFWKSINS KNYFTSAAYP ELFIATKEQS RVHLARGLPS MTDFQIS

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipette the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.