Recombinant Mouse IL-1 beta

Molecule:

IL-1 beta

Synonyms:

Il-1b, IL-1beta

Species:

Mouse

Cat. No.:

CRI139B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$185.00 BUY

Description:

Interleukin-1 beta (IL-1beta) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1beta is a secreted cytokine, IL-1alpha is predominantly a cell associated cytokine.

Uniprot Name:

Interleukin-1 beta

Uniprot ID:

P10749

Data PDF:

CRI139

Source:

E. coli

Molecular Weight:

17.5 kDa (153 aa)

Formulation:

Lyophilized from a 0.2 μm filtered 10 mM sodium phosphate, pH 7.5.

Purity:

≥ 95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 < 15 pg/ml, determined by a cell proliferation assay of mouse D10S cells.

Specific Activity:

≥ 6.7 × 10e7 IU/mg.

Endotoxin Level:

≤ 1 EU/μg as determined by kinetic LAL method.

Amino Acid Sequence:

MVPIRQLHYR LRDEQQKSLV LSDPYELKAL HLNGQNINQQ VIFSMSFVQG EPSNDKIPVA LGLKGKNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKSK VEFESAEFPN WYISTSQAEH KPVFLGNNSG QDIIDFTMES VSS

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.