Recombinant Mouse IL-10

Molecule:

IL-10

Synonyms:

B-TCGF, CSIF, TGIF, IL10A, MGC126450, MGC126451, Cytokine Synthesis Inhibitory Factor

Species:

Mouse

Cat. No.:

CRI154C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$4,820.00 / Special Price: $3,720.00 BUY

Description:

Recombinant Mouse Interleukin-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophanges, monocytes, T cells, B cells and keratinocytes. IL10 inhibits the expression of proinflammatory cytokines such as IL1 and TNF-alpha. Like IL4, IL10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL10 is active on mouse cells, but mouse IL10 is inactive on human cells.

Entrez Symbol:

Il10

Uniprot Name:

Interleukin-10

Uniprot ID:

P18893

Data PDF:

CRI154

Source:

E. coli

Molecular Weight:

Noncovalent homodimer, 18.9 kDa (161 aa)

Physical State:

Lyophilized

Formulation:

Sterile filtered solution containing 10 mM phosphate, pH 7.5

Purity:

>95% by Reducing and Non-reducing SDS-PAGE

Biological Activity:

ED50 ≤ 5 ng/ml, determined by the dose-dependent proliferation of mouse MC/9 cells.

Specific Activity:

≥ 2.0 x 10e5 U/mg

Endotoxin Level:

< 1.0 EU/µg by kinetic LAL

Amino Acid Sequence:

MSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGQKLK TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLEDQGVYKA MNEFDIFINC IEAYMMIKMK S

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in 10 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Store as supplied at -20 °C to -80 °C for up to 1 year.