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Recombinant Mouse IL-10 Animal Free

Molecule:

IL-10

Synonyms:

IL-10, Cytokine Synthesis Inhibitory Factor, CSIF

Species:

Mouse

Cat. No.:

CRI154C-AF pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$5,300.00 / Special Price: $4,450.00 BUY

Description:

Recombinant Mouse Interleukin-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophanges, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF-alpha. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10 is active on mouse cells, but mouse IL-10 is inactive on human cells.

Manufactured in an Animal free facility without animal-devrved products.

Entrez Symbol:

Il10

Uniprot Name:

Interleukin-10

Uniprot ID:

P18893

Data PDF:

CRI154-AF

Source:

E. coli

Molecular Weight:

18.9 kDa (161 aa) noncovalent homodimer

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile filtered solution containing 10 mM sodium phosphate, pH 7.5

Purity:

>95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50≤ 5 ng/ml, determined by dose-dependent proliferation of mouse MC/9 cells.

Specific Activity:

≥ 2.0 x 10e5 U/mg

Endotoxin Level:

< 1.0 EU/µg by kinetic LAL analysis

Amino Acid Sequence:

MSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGQKLK TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLEDQGVYKA MNEFDIFINC IEAYMMIKMK S

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipette the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.