Recombinant Mouse Interleukin-12 p70

Molecule:

IL-12

Synonyms:

IL-12, IL-12a, IL-12b, p35, p40

Species:

Mouse

Cat. No.:

CRI264B pdf (datasheet)

Quantity/Size:

10 µg

Price:

$450.00 BUY

Description:

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

Entrez Symbol:

il12a, il12b

Uniprot Name:

Interleukin-12

Uniprot ID:

P43431, P43432

Data PDF:

CRI264

Source:

CHO cells

Molecular Weight:

75 kDa heterodimer composed of 35 kDa and 40 kDa subunits

Formulation:

Lyophilized from PBS

Purity:

> 98% by SDS-PAGE and HPLC

Biological Activity:

Assay #1: ED50 < 0.1 ng/ml, determined by the stimulation of IFN-gamma production by murine splenocytes co-stimulated with IL-18.


Assay #2: ED50 is ≤ 0.1 ng/ml, etermined by its ability to stimulate the proliferation of 2D6 cells.

Specific Activity:

Assay #1: > 1 x 107 units/mg
Assay #2: > 1 x 107 units/mg

Endotoxin Level:

≤ 1 EU/μg protein

Storage/Stability:

Store in working aliquots at -80°C. Avoid repeated freeze-thaw cycles.