Recombinant Mouse IL-13

Molecule:

IL-13

Synonyms:

IL-13, T-cell activation protein P600

Species:

Mouse

Cat. No.:

CRI159B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$185.00 BUY

Description:

Interleukin-13 (IL-13) is an immunoregulatory cytokine produced primarily by activated Th2 cells, and also by mast cells and NK cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1beta, TNF-alpha, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching resulting in increased production of IgE. Blocking of IL-13 activity inhibits the pathophysiology of asthma.IL-13 functions are mediated through the JAK/STAT signaling pathway. Human and mouse IL-13 are crossreactive.

Uniprot Name:

Interleukin-13

Uniprot ID:

P20109

Data PDF:

CRI159

Source:

E. coli

Molecular Weight:

12.3 kDa (111 aa) monomer

Formulation:

Lyophilized from a sterile filtered aqueous solution containing 0.1% Trifluoroacetic Acid.

Purity:

≥ 95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 ≤ 10 ng/ml, determined by a cell proliferation assay using the TF-1 cell line.

Specific Activity:

≥ 1.0 × 10e5 U/mg

Endotoxin Level:

≤ 1 EU/μg determined by kinetic LAL analysis.

Amino Acid Sequence:

MPVPRSVSLP LTLKELIEEL SNITQDQTPL CNGSMVWSVD LAAGGFCVAL DSLTNISNCN AIYRTQRILH GLCNRKAPTT VSSLPDTKIE VAHFITKLLS YTKQLFRHGP FMPVPRSVSLP LTLKELIEEL SNITQDQTPL CNGSMVWSVD LAAGGFCVAL DSLTNISNCN AIYRTQRILH GLCNRKAPTT VSSLPDTKIE VAHFITKLLS YTKQLFRHGP F

Reconstitution:

Centrifuge vial prior to opening. Add sterile 20 mM HCl to a concentration of 0.1 mg/mL and gently pipet the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.