Synonyms:
IL-2, lymphokine, aldesleukin, T cell growth factor
Description:
Interleukin-2 (IL-2) is an immunomodulatory cytokine that is produced by lymphocytes. IL
-2 signals through the IL-2R receptor to induce activated T cell proliferation and promote
T cell differentiation. IL-2 also stimulates the proliferation and differentiation of B cells,
natural killer cells, monocytes, and macrophages. IL-2 plays a central role in the
expansion and maintenance of regulatory T cells, although it inhibits the development of
Th17 polarized cells.
Uniprot Name:
Interleukin-2
Molecular Weight:
Monomer, 17.4 kDa (150 aa)
Physical State:
Lyophilized
Formulation:
Lyophilized from sterile filtered aqueous solution containing 10 mM sodium citrate, pH 4.0.
Purity:
≥95% by nonreducing and reducing SDS-PAGE
Biological Activity:
ED50 < 5 ng/ml, as determined by proliferation assay of murine CTLL-2 cells.
Specific Activity:
≥ 2.0 x 105 U/mg calibrated against recombinant mouse IL-2 WHO International Standard (NIBSC code: 93/566).
Endotoxin Level:
≤ 1 EU/µg, by kinetic LAL
Amino Acid Sequence:
MAPTSSSTSS STAEAQQQQQ QQQQQQQHLE QLLMDLQELL SRMENYRNLK LPRMLTFKFY LPKQATELKD LQCLEDELGP LRHVLDLTQS KSFQLEDAEN FISNIRVTVV KLKGSDNTFE CQFDDESATV VDFLRRWIAF CQSIISTSPQ
Reconstitution:
Centrifuge vial prior to opening. Add sterile 10mM acetic acid to a concentration of 0.1 mg/mL. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.
Storage/Stability:
Store as supplied at -20 °C to -80 °C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots containing 0.1% BSA at -20 °C to -80 °C. Avoid repeated freeze-thaw cycles.
