Synonyms:
interleukin-2, aldesleukin, T cell growth factor, involved in regulation of T-cell clonal expansion
Description:
Recombinant Mouse IL-2 (IL-2) is a single, non-glycosylated polypeptide chain containing 150 amino acids.
Background: Interleukin-2 (IL-2) is an immunomodulatory cytokine that is produced by lymphocytes. IL-2 signals through the IL-2R receptor to induce activated T cell proliferation and promote T cell differentiation. IL-2 also stimulates the proliferation and differentiation of B cells, natural killer cells, monocytes, and macrophages. The receptor for IL-2 consists of three subunits that are present on the cell surface in varying pre-formed complexes . The 55 kDa IL-2 Rα is specific for IL-2 and binds with low affinity. The 75 kDa IL-2 Rβ, which is also a component of the IL-15 receptor, binds IL-2 with intermediate affinity. The 64 kDa common gamma chain receptor transduces the signal. IL-2 contributes to T cell homeostasis by promoting the Fas-induced death of naïve CD4+ T cells but not activated CD4+ memory lymphocytes . IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells.
Uniprot Name:
Interleukin-2
Molecular Weight:
17.4 kDa
Physical State:
Lyophilized
Formulation:
Lyophilized from a sterile-filtered aqueous solution containing 10 mM sodium citrate, pH 4.0.
Biological Activity:
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 5 ng/ml.
Specific Activity:
> 2.0 x 105 U/mg calibrated against recombinant mouse IL-2 WHO International Standard (NIBSC code: 93/566).
Endotoxin Level:
≤1 EU/µg of protein by kinetic LAL analysis
Amino Acid Sequence:
MAPTSSSTSS STAEAQQQQQ QQQQQQQHLE QLLMDLQELL SRMENYRNLK LPRMLTFKFY LPKQATELKD LQCLEDELGP LRHVLDLTQS KSFQLEDAEN FISNIRVTVV KLKGSDNTFE CQFDDESATV VDFLRRWIAF CQSIISTSPQ
Reconstitution:
Centrifuge vial prior to opening. Add sterile 10mM acetic acid to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.
Storage/Stability:
Upon receipt, store desiccated at -20°C for up to 1 year. After reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C.
