Recombinant Mouse IL-2

Molecule:

IL-2

Synonyms:

IL-2, lymphokine, aldesleukin, T cell growth factor

Species:

Mouse

Cat. No.:

CRI145B pdf (datasheet)

Quantity/Size:

20 μg

Price:

$185.00 BUY

Description:

Interleukin-2 (IL-2) is an immunomodulatory cytokine that is produced by lymphocytes. IL -2 signals through the IL-2R receptor to induce activated T cell proliferation and promote T cell differentiation. IL-2 also stimulates the proliferation and differentiation of B cells, natural killer cells, monocytes, and macrophages. IL-2 plays a central role in the expansion and maintenance of regulatory T cells, although it inhibits the development of Th17 polarized cells.

Entrez Symbol:

Il2

Uniprot Name:

Interleukin-2

Uniprot ID:

P04351

Data PDF:

CRI145

Source:

E. coli

Molecular Weight:

Monomer, 17.4 kDa (150 aa)

Physical State:

Lyophilized

Formulation:

Lyophilized from sterile filtered aqueous solution containing 10 mM sodium citrate, pH 4.0.

Purity:

≥95% by nonreducing and reducing SDS-PAGE

Biological Activity:

ED50 < 5 ng/ml, as determined by proliferation assay of murine CTLL-2 cells.

Specific Activity:

≥ 2.0 x 105 U/mg calibrated against recombinant mouse IL-2 WHO International Standard (NIBSC code: 93/566).

Endotoxin Level:

≤ 1 EU/µg, by kinetic LAL

Amino Acid Sequence:

MAPTSSSTSS STAEAQQQQQ QQQQQQQHLE QLLMDLQELL SRMENYRNLK LPRMLTFKFY LPKQATELKD LQCLEDELGP LRHVLDLTQS KSFQLEDAEN FISNIRVTVV KLKGSDNTFE CQFDDESATV VDFLRRWIAF CQSIISTSPQ

Reconstitution:

Centrifuge vial prior to opening. Add sterile 10mM acetic acid to a concentration of 0.1 mg/mL. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Store as supplied at -20 °C to -80 °C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots containing 0.1% BSA at -20 °C to -80 °C. Avoid repeated freeze-thaw cycles.