Recombinant Mouse Interleukin-21

Molecule:

IL-21

Synonyms:

IL-21

Species:

Mouse

Cat. No.:

CS322A pdf (datasheet)

Quantity/Size:

2 μg

Price:

$85.00 BUY

Description:

Interleukin-21 (IL-21) is a member of the common gamma-chain family of cytokines (includes IL-2, IL-4, IL-7, IL-9, IL-13, and IL-15) and is expressed by activated CD4+ T cells. IL-21 has a private receptor known as IL-21R, which is expressed on T, B, and NK cells. Within the B cell lineage, IL-21 regulates IgG1 production and cooperates with IL-4 for the production of multiple Ab classes in vivo. Initial studies have demonstrated that IL-21 has pleiotropic effects on the proliferation, differentiation, and effector functions of B, T, natural killer, and dendritic cells.

Entrez Symbol:

Il21

Uniprot Name:

Interleukin-21

Uniprot ID:

Q9ES17

Data PDF:

CS322

Source:

E. coli

Molecular Weight:

15.1 kDa (130 aa)

Formulation:

Lyophilized from a sterile filtered solution containing 20 mM NaHCO3, pH 8.5

Purity:

≥ 95% determined by reducing and non-reducing SDS-PAGE.

Biological Activity:

ED50 ≤20 ng/ml, determined by a dose-dependent proliferation of B9 cells.< 20 ng/ml, determined by a dose-dependent proliferation of B9 cells.< 20 ng/ml, determined by a dose-dependent proliferation of B9 cells.

Specific Activity:

≥5.0 x 104 units/mg

Endotoxin Level:

≤ 1 EU/μg by kinetic LAL analysis.

Amino Acid Sequence:

MHKSSPQGPD RLLIRLRHLI DIVEQLKIYE NDLDPELLSA PQDVKGHCEH AAFACFQKAK LKPSNPGNNK TFIIDLVAQL RRRLPARRGG KKQKHIAKCP SCDSYEKRTP KEFLERLKWL LQKMIHQHLS

Reconstitution:

Centrifuge vial before opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile water at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.

Storage/Stability:

Upon receipt, store at -20°C to -80°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, prepare working aliquots and store at -20 to -80°C. For maximal stability, dilute to working aliquots in a 0.1% BSA solution. Avoid repeated freeze-thaw cycles.