Recombinant Mouse IL-4

Molecule:

IL-4

Synonyms:

Interleukin-4, BSF-1, IL-4, B cell Stimulating Factor, BCDF, BCGF

Species:

Mouse

Cat. No.:

CRI129B pdf (datasheet)

Quantity/Size:

20 μg

Price:

$185.00 BUY

Description:

Interleukin-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy.

Entrez Symbol:

Il4

Uniprot Name:

Interleukin-4

Uniprot ID:

P07750

Data PDF:

CRI129

Source:

E. coli

Molecular Weight:

13.7 kDa (121 aa)

Physical State:

Lyophilized

Formulation:

Sterile-filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA).

Purity:

>95%, by reducing and non-reducing SDS-PAGE.

Biological Activity:

ED50 < 20 ng/ml, as determined by the dose-dependent proliferation of Mouse HT-2 cells calibrated against recombinant mouse IL-4 WHO International Standard (NIBSC code: 91/656). .

Specific Activity:

≥ 5 x 10e4 IU/mg

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis

Amino Acid Sequence:

MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Store at -20°C to -80°C for up to 1 year.