Synonyms:
IL-7, Lymphopoietin 1 (LP-1), pre-B cell Factor
Description:
Interleukin-7 (IL-7) is encoded by the IL7 gene and secreted by stromal cells in the red
marrow and thymus. IL-7 binds to the IL-7 receptor, a heterodimer consisting of IL-7
receptor alpha and IL-2 receptor gamma chain. IL-7 stimulates the differentiation of
hematopoietic stem cells into lymphoid progenitor cells. It also stimulates proliferation of
B cells, T cells and NK cells. Mouse IL-7 has approximately 65% and 88% amino acid
sequence identity with human and rat IL-7 and both proteins exhibit cross-species
activity. IL-7 as an immunotherapy agent has been examined in many human clinical
trials for various malignancies and during HIV infection.
Uniprot Name:
Interleukin-7
Molecular Weight:
15 kDa (130 aa) monomer
Formulation:
Lyophilized from a sterile filtered solution in 10 mM acetic acid
Purity:
≥ 95% by reducing and non-reducing SDS-PAGE
Biological Activity:
≤ 1 ng/ml determined by dose-dependent 2E8 cell proliferation
Specific Activity:
≥ 1.0 x 10e6 IU/mg.
Endotoxin Level:
≤ 1 EU/μg as determined by kinetic LAL analysis
Amino Acid Sequence:
MECHIKDKEG KAYESVLMIS IDELDKMTGT DSNCPNNEPN FFRKHVCDDT KEAAFLNRAA RKLKQFLKMN ISEEFNVHLL TVSQGTQTLV NCTSKEEKNV KEQKKNDACF LKRLLREIKT CWNKILKGSI
Reconstitution:
Centrifuge vial prior to opening. Add sterile 10 mM acetic acid to a concentration of 0.1 mg/ml. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.
Storage/Stability:
Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.
