Recombinant Mouse IL-7

Molecule:

IL-7

Synonyms:

IL-7, Lymphopoietin 1 (LP-1), pre-B cell Factor

Species:

Mouse

Cat. No.:

CRI131A pdf (datasheet)

Quantity/Size:

2 μg

Price:

$85.00 BUY

Description:

Interleukin-7 (IL-7) is encoded by the IL7 gene and secreted by stromal cells in the red marrow and thymus. IL-7 binds to the IL-7 receptor, a heterodimer consisting of IL-7 receptor alpha and IL-2 receptor gamma chain. IL-7 stimulates the differentiation of hematopoietic stem cells into lymphoid progenitor cells. It also stimulates proliferation of B cells, T cells and NK cells. Mouse IL-7 has approximately 65% and 88% amino acid sequence identity with human and rat IL-7 and both proteins exhibit cross-species activity. IL-7 as an immunotherapy agent has been examined in many human clinical trials for various malignancies and during HIV infection.

Uniprot Name:

Interleukin-7

Uniprot ID:

P10168

Data PDF:

CRI131

Source:

E. coli

Molecular Weight:

15 kDa (130 aa) monomer

Formulation:

Lyophilized from a sterile filtered solution in 10 mM acetic acid

Purity:

≥ 95% by reducing and non-reducing SDS-PAGE

Biological Activity:

≤ 1 ng/ml determined by dose-dependent 2E8 cell proliferation

Specific Activity:

≥ 1.0 x 10e6 IU/mg.

Endotoxin Level:

≤ 1 EU/μg as determined by kinetic LAL analysis

Amino Acid Sequence:

MECHIKDKEG KAYESVLMIS IDELDKMTGT DSNCPNNEPN FFRKHVCDDT KEAAFLNRAA RKLKQFLKMN ISEEFNVHLL TVSQGTQTLV NCTSKEEKNV KEQKKNDACF LKRLLREIKT CWNKILKGSI

Reconstitution:

Centrifuge vial prior to opening. Add sterile 10 mM acetic acid to a concentration of 0.1 mg/ml. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.