Recombinant Mouse Leptin

Molecule:

Leptin

Synonyms:

OB protein, Obesity protein, OBS, Obesity factor

Species:

Mouse

Cat. No.:

CRL301C pdf (datasheet)

Quantity/Size:

5 mg

Price:

$520.00 BUY

Description:

Leptin is a protein that is thought to have a critical role in the physiologic regulation of body weight via its capacity to inhibit food intake and stimulate energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans can be caused by defects in leptin production. These functions include the regulation of hematopoiesis, angiogenesis, wound healing, inflammation, and immune responses.

Entrez Symbol:

Lep

Uniprot Name:

Leptin

Uniprot ID:

P41160

Data PDF:

CRL301

Source:

E. coli

Molecular Weight:

16.1 kDa (147 aa) monomer

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5

Purity:

≥ 95.0% by Reducing and Non-reducing SDS-PAGE

Biological Activity:

This product demonstrates dose-dependent cell proliferation using Ba/F3 cells

Endotoxin Level:

≤ 1 EU/μg by kinetic LAL analysis

Amino Acid Sequence:

MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC

Reconstitution:

Centrifuge vial prior to opening. When reconstituting the product, gently pipet and wash down the sides of the vial to ensure full recovery of the protein into solution. It is recommended to reconstitute the lyophilized product with sterile 20 mM HCl at a concentration of 0.1 mg/mL, which can be further diluted into other aqueous solutions.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.