Recombinant Mouse LIF

Molecule:

LIF

Synonyms:

Recombinant Mouse LIF, Leukemia inhibitory factor, Lif

Species:

Mouse

Cat. No.:

CSI20150A pdf (datasheet)

Quantity/Size:

5 μg

Price:

$85.00 BUY

Description:

Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines, and promotion of megakaryocyte production in vivo.

Entrez Symbol:

Lif

Uniprot Name:

Leukemia inhibitory factor

Uniprot ID:

P09056

Data PDF:

CSI20150

Source:

E. coli

Molecular Weight:

20 kDa (181 aa) monomer

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA).

Purity:

≥ 95% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 < 1.0 ng/ml, determined by dose-dependent induction of IL-6 in mouse M1 cells.

Specific Activity:

≥ 1.0 x 10e6 units/mg

Endotoxin Level:

≤1 EU/µg by kinetic LAL analysis

Amino Acid Sequence:

MSPLPITPVN ATCAIRHPCH GNLMNQIKNQ LAQLNGSANA LFISYYTAQG EPFPNNVEKL CAPNMTDFPS FHGNGTEKTK LVELYRMVAY LSASLTNITR DQKVLNPTAV SLQVKLNATI DVMRGLLSNV LCRLCNKYRV GHVDVPPVPD HSDKEAFQRK KLGCQLLGTY KQVISVVVQA F

Reconstitution:

Centrifuge vial prior to opening. Add sterile 10 mM acetic acid to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Upon receipt, store at -20°C to -80°C for up to 1 year.