Synonyms:
Macrophage Colony Stimulating Factor, CSF-1
Description:
Macrophage Colony Stimulating Factor (M-CSF) is a hematopoietic cytokine/ growth factor produced by a wide variety of cells. M-CSF controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. M-CSF acts through the CSF receptor 1.
Molecular Weight:
Dimer, 18.2/36.4 kDa (156/312 aa)
Physical State:
Lyophilized
Formulation:
Lyophilized from a sterile-filtered aqueous solution containing 10mM sodium phosphate, 50 mM sodium chloride, pH 7.5.
Purity:
> 95% by reducing and non-reducing SDS-PAGE
Biological Activity:
ED50 < 10 ng/ml, determined by dose-dependent proliferation of M-NFS-60 cells.
Specific Activity:
≥ 1.0 x 10e5 U/mg
Endotoxin Level:
< 1 EU/µg of protein by kinetic LAL
Amino Acid Sequence:
MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP
Storage/Stability:
Store as supplied at -20°C to -80°C for up to 1 year.
