Synonyms:
Macrophage migration inhibitory factor, delayed early response protein 6, DER6, glycosylation inhibiting factor, GIF, L-dopamine isomerase.
Description:
Macrophage migration inhibitory factor (MIF) is a pro-inflammatory lymphokine that functions during cell-mediated immmunity. MIF promotes fibroblast migration by inducing interleukin 1 (IL-1), interleukin 8 (IL-8), and matrix metalloproteinase (MMP) expression. In interferon-gamma-activated macrophages, MIF stimulates nitric oxide (NO) production and tumor necrosis factor alpha (TNF-α) secretion.
Uniprot Name:
Macrophage migration inhibitory factor
Molecular Weight:
Monomer, 12.5 kDa (115 aa)
Physical State:
Lyophilized
Formulation:
Sterile-filtered solution containing 10 mM sodium phosphate, pH 7.5
Purity:
≥95% by reducing and non-reducing SDS-PAGE
Biological Activity:
Activity determined by ERK pathway activation in RAW cells, detected via Western Blot
Endotoxin Level:
≤1 EU/µg by kinetic LAL analysis
Amino Acid Sequence:
MPMFIVNTNV PRASVPEGFL SELTQQLAQA TGKPAQYIAV HVVPDQLMTF SGTNDPCALC SLHSIGKIGG AQNRNYSKLL CGLLSDRLHI SPDRVYINYY DMNAANVGWN GSTFA
Storage/Stability:
Store as supplied at -20°C to -80°C for up to 1 year.
