Synonyms:
Kit ligand, Hematopoietic growth factor KL, Mast cell growth factor, MGF, Steel factor, Stem cell factor, SCF, c-Kit ligand
Description:
Stem cell factor (SCF) also known as mast cell growth factor (MGF) and steel-factor
(SLF), plays essential roles in gametogenesis, melanogenesis and early stages of
hematopoiesis. In vitro and in vivo, SCF can stimulate the proliferation of mature, as well
as the proliferation and maturation of immature, mast cells. On purified primitive human
and mouse hematopoietic precursors, SCF acts in a synergistic manner with various
growth factors, such as IL-1, IL-3, IL-6, IL-7, and Epo, to induce myeloid, erythroid and
lymphoid lineage colony formation. While human SCF shows no activity on murine cells,
murine and rat SCF are active on human cells.
Molecular Weight:
18.4 kDa predicted (165 aa), monomer
Formulation:
Lyophilized from sterile-filtered 20 mM sodium phosphate, pH 6.5
Purity:
≥ 95% by reducing and non-reducing SDS-PAGE
Biological Activity:
≤ 20 ng/ml, determined by the dose-dependent proliferation of human TF-1 cells.
Specific Activity:
≥5.0 x 104 U/mg
Endotoxin Level:
≤1 EU/μg as determined kinetic LAL analysis
Amino Acid Sequence:
MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipette the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.
Storage/Stability:
Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, store at 2-8°C for up to 1 month or prepare working aliquots and store at -20°C to -80°C for up to 3 months. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.
