Recombinant Mouse TNF-alpha Animal Free

Synonyms:

TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, Cachectin

Cat. No.:

CRT192C-AF pdf (datasheet)

Quantity/Size:

1 mg

Price:

$3,520.00 / Special Price: $2,300.00 BUY

Description:

Tumor necrosis factor alpha (TNF-alpha) is a multifunctional proinflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, NK-cells following their stimulation by bacterial LPS. TNF-alpha activates signals through two receptors, TNFR1, which is expressed on most cell types, and TNFR2, which is expressed mainly on immune cells. TNF-alpha can have many functions, including stimulation of phagocytosis in macrophages, chemoattraction of neutrophils, increase in insulin resistance, and inducing fever, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.

Manufactured without Animal-derived products, in an Animal Free facility.

Uniprot ID:

P06804

Data PDF:

CRT192-AF

Source:

E. coli

Molecular Weight:

Monomer, 17.4 kDa (157 aa)

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile filtered solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.

Purity:

>95% by reducing and non-reducing SDS-PAGE

Biological Activity:

Typical ED50 < 20 pg/ml, determined by cytolysis of mouse L929 cells in the presence of Actinomycin D.

Specific Activity:

> 1.0 × 10e7 U/mg

Endotoxin Level:

< 1 EU/μg by kinetic LAL analysis

Amino Acid Sequence:

MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipette the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Store as supplied at -20 °C to -80 °C for up to one year.