TNF-alpha

TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, Cachectin

Mouse

CRT192C-AF pdf (datasheet)

1 mg

$1,690.00 / Special Price: $1,320.00 BUY

Tumor necrosis factor alpha (TNF-alpha) is a multifunctional proinflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, NK-cells following their stimulation by bacterial LPS. TNF-alpha activates signals through two receptors, TNFR1, which is expressed on most cell types, and TNFR2, which is expressed mainly on immune cells. TNF-alpha can have many functions, including stimulation of phagocytosis in macrophages, chemoattraction of neutrophils, increase in insulin resistance, and inducing fever, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.

Manufactured without Animal-derived products, in an Animal Free facility.

Tumor necrosis factor

P06804

CRT192-AF

E. coli

Monomer, 17.4 kDa (157 aa)

Lyophilized

Lyophilized from a sterile filtered solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.

≥95% by reducing and non-reducing SDS-PAGE

Typical ED50 < 20 pg/ml, determined by cytolysis of mouse L929 cells in the presence of Actinomycin D.

≥ 1.0 × 10⁷ U/mg

≤ 1 EU/μg by kinetic LAL analysis

MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipette the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Store as supplied at -20 °C to -80 °C for up to one year. Upon reconstitution the preparation is stable for up to one month at 2- 8 °C. For long term, reconstitute in working aliquots containing 0.1 % BSA and store at -80 °C. Avoid repeated freeze-thaw cycles.