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Recombinant Mouse TNF-alpha

Molecule:

TNF-alpha

Synonyms:

TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, Cachectin

Species:

Mouse

Cat. No.:

CRT192B pdf (datasheet)

Quantity/Size:

20 μg

Price:

$185.00 BUY

Description:

Recombinant Mouse TNF-alpha is a soluble 157 amino acid protein which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

Background: Tumor necrosis factor alpha (TNF-alpha) is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. Mouse TNF-alpha occurs as a membrane-anchored form. The naturally-occurring form of TNF-alpha is glycosylated, but non-glycosylated recombinant TNF-alpha has comparable biological activity. The biologically active native form of TNF-alpha is reportedly a trimer. Human and mouse TNF-alpha show approximately 79% homology at the amino acid level and cross reactivity between the two species.

Entrez Symbol:

Tnf

Uniprot Name:

tumor necrosis factor

Uniprot ID:

P06804

Data PDF:

CRT192

Source:

E. coli

Molecular Weight:

Monomer, 17.4 kDa (157 aa)

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile-filtered solution containing 10 mM sodium phosphate and 50 mM sodium chloride, pH 7.5.

Purity:

>95% by reducing and non-reducing SDS-PAGE

Biological Activity:

Typical ED50 < 20 pg/ml, determined by cytolysis of mouse L929 cells in the presence of Actinomycin D.

Specific Activity:

> 1.0 × 10e7 U/mg

Endotoxin Level:

< 1 EU/μg by kinetic LAL analysis

Amino Acid Sequence:

MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Store as supplied at -20 °C to -80 °C for up to one year.