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Recombinant Mouse TNF-alpha

Molecule:

TNF-alpha

Synonyms:

TNF-alpha, TNFA, TNFSF2, Tnfa, Tnfsf1a, tumor necrosis factor, TNF-a, cachectin

Species:

Mouse

Cat. No.:

CRT192B pdf (datasheet)

Quantity/Size:

20 μg

Price:

$235.00 BUY

Description:

Recombinant Mouse TNF-alpha is a soluble 157 amino acid protein which corresponds to C-terminal extracellular domain of the full length transmembrane protein.

Background: Tumor necrosis factor alpha (TNF-alpha) is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. Mouse TNF-alpha occurs as a membrane-anchored form. The naturally-occurring form of TNF-alpha is glycosylated, but non-glycosylated recombinant TNF-alpha has comparable biological activity. The biologically active native form of TNF-alpha is reportedly a trimer. Human and mouse TNF-alpha show approximately 79% homology at the amino acid level and cross reactivity between the two species.

Entrez Symbol:

Tnf

Uniprot Name:

tumor necrosis factor

Uniprot ID:

P06804

Data PDF:

CRT192

Source:

E. coli

Molecular Weight:

~17.4 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile-filtered solution containing 10 mM sodium phosphate and 50 mM sodium chloride, pH 7.5.

Purity:

>95% by SDS-PAGE

Biological Activity:

As determined by a cytotoxicity assay using mouse L929 cells in the presence of Actinomycin D, is <0.1 ng/ml.

Specific Activity:

>1.0 × 107 units/mg

Endotoxin Level:

<1 EU/μg as determined by LAL method.

Amino Acid Sequence:

MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Lyophilized product is stable at room temperature for shipping purposes. Upon receipt, store desiccated at -20°C for up to 1 year. Upon reconstitution, the preparation is stable for up to one month at 2-8°C. For long term storage, freeze in working aliquots and store at -20 to -80°C. For maximal stability, dilute to working aliquots in a 0.1% BSA solution. Avoid repeated freeze-thaw cycles.