Recombinant Mouse TNF-alpha

Molecule:

TNF-alpha

Synonyms:

TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, Cachectin

Species:

Mouse

Cat. No.:

CRT192B pdf (datasheet)

Quantity/Size:

20 μg

Price:

$185.00 BUY

Description:

Tumor necrosis factor alpha (TNF-alpha) is a multifunctional proinflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, NK-cells following their stimulation by bacterial LPS. TNF-alpha activates signals through two receptors, TNFR1, which is expressed on most cell types, and TNFR2, which is expressed mainly on immune cells. TNF-alpha can have many functions, including stimulation of phagocytosis in macrophages, chemoattraction of neutrophils, increase in insulin resistance, and inducing fever, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation.

Entrez Symbol:

Tnf

Uniprot Name:

Tumor necrosis factor

Uniprot ID:

P06804

Data PDF:

CRT192

Source:

E. coli

Molecular Weight:

Monomer, 17.4 kDa (157 aa)

Formulation:

Lyophilized from a sterile filtered solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.

Purity:

≥95% by reducing and non-reducing SDS-PAGE

Biological Activity:

Typical ED50 < 20 pg/ml, determined by cytolysis of mouse L929 cells in the presence of Actinomycin D.

Specific Activity:

≥ 1.0 × 107 U/mg

Endotoxin Level:

≤ 1 EU/μg by kinetic LAL analysis

Amino Acid Sequence:

MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipette the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Store, as supplied, for up to 1 year at -20°C to -80°C Reconstituted protein is stable for one week at 4°C. For long term storage, aliquot and store at -20°C to -80°C with a carrier protein such as 0.1% HSA or BSA as a stabilizer. This depends upon the particular application employed. Avoid repeated freeze-thaw cycles.