Synonyms:
TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, Cachectin
Description:
Tumor necrosis factor alpha (TNF-alpha) is a multifunctional proinflammatory cytokine
secreted by macrophages, monocytes, neutrophils, T cells, NK-cells following their
stimulation by bacterial LPS. TNF-alpha activates signals through two receptors, TNFR1,
which is expressed on most cell types, and TNFR2, which is expressed mainly on
immune cells. TNF-alpha can have many functions, including stimulation of phagocytosis
in macrophages, chemoattraction of neutrophils, increase in insulin resistance, and
inducing fever, cell proliferation, differentiation, apoptosis, lipid metabolism, and
coagulation.
Uniprot Name:
Tumor necrosis factor
Molecular Weight:
Monomer, 17.4 kDa (157 aa)
Physical State:
Lyophilized
Formulation:
Lyophilized from a sterile filtered solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.
Purity:
≥95% by reducing and non-reducing SDS-PAGE
Biological Activity:
Typical ED50 < 20 pg/ml, determined by cytolysis of mouse L929 cells in the presence of Actinomycin D.
Specific Activity:
≥ 1.0 × 107 U/mg
Endotoxin Level:
≤ 1 EU/μg by kinetic LAL analysis
Amino Acid Sequence:
MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipette the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.
Storage/Stability:
Store as supplied at -20 °C to -80 °C for up to one year. Upon reconstitution the preparation is stable for up to one month at 2- 8 °C. For long term, reconstitute in working aliquots containing 0.1 % BSA and store at -80 °C. Avoid repeated freeze-thaw cycles.
