Synonyms:
Interleukin-2, aldesleukin, T cell growth factor, involved in regulation of T-cell clonal expansion
Description:
Interleukin 2 (IL-2) is an immunomodulatory cytokine that is produced by lymphocytes. IL
-2 signals through the IL-2R receptor to induce activated T cell proliferation and promote
T cell differentiation. IL-2 also stimulates the proliferation and differentiation of B cells,
natural killer cells, monocytes, and macrophages.
Uniprot Name:
Interleukin-2
Molecular Weight:
15.3 kDa (135 aa)
Formulation:
Sterile filtered through a 0.22 micron filter then lyophilized, carrier free.
Purity:
>95% as determined by SDS-PAGE. Purified via affinity chromatography.
Biological Activity:
ED50 < 1.0 ng/ml, determined by dose-dependent proliferation of mouse CTLL-2 cells. The optimal concentration for each specific application should be determined by an initial dose-response assay.
Specific Activity:
1.0 x 10e6 units/ml.
Endotoxin Level:
<0.1 ng/µg of protein
Amino Acid Sequence:
MAPTSSSTKN TKKQLEPLLL DLQLLLKEVK NYENADLSRM LTFKFYMPKQ ATELKHLQCL VEELKALEGV LNLGQSKNSD SANIKESMNN INVTVLELKG SETSFKCEYD DETVTAVEFL NKWITFCQSI YSTLT
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipet the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for reconstitution. A small amount of precipitate may be seen.
Storage/Stability:
Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.
