Recombinant Protein G, His Tag

Synonyms:

Immunoglobulin G-binding protein G

Cat. No.:

CRG133C pdf (datasheet)

Quantity/Size:

1 g

Price:

$2,995.00 BUY

Description:

Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology. Recombinant Protein G has a C-terminal His tag.

Entrez Symbol:

spg

Uniprot Name:

Immunoglobulin G-binding protein G

Uniprot ID:

P19909

Data PDF:

CRG133

Source:

E. coli

Molecular Weight:

Predicted MW of ~21 kDa, Apparent MW of ~29 kDa in SDS-PAGE

Formulation:

Lyophilized from a sterile-filtered solution.

Purity:

>90% by SDS-PAGE

Endotoxin Level:

< 0.1EU/ug

Reconstitution:

Centrifuge vial prior to opening. Dissolve in distilled water or saline.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution as directed, stable for up to 1 month at 2-8°C or prepare working aliquots and store at -20°C to -80°C for up to 3 months. Avoid repeated freeze-thaw cycles.