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Recombinant Rat CXCL12/SDF-1 alpha

Molecule:

CXCL12

Synonyms:

SDF1, SDF1A, SDF1B, Sdf1

Species:

Rat

Cat. No.:

CSI20133B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$235.00 BUY

Description:

Recombinant Rat CXCL12/SDF-1 alpha is a single non-glycosylated polypeptide chain containing 68 amino acids.

Background: SDF-1 alpha and SDF-1 beta, members of the chemokine alpha subfamily that lack the ELR domain, were initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. These proteins were subsequently also cloned from a human stromal cell line as cytokines that supported the proliferation of a stromal cell-dependent pre-B-cell line. SDF-1 alpha and SDF-1 beta cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. Both SDF-1 alpha and SDF-1 beta are encoded by a single gene and arise by alternative splicing.  The two proteins are identical except for the four amino acid residues that are present in the carboxy-terminus of SDF-1 beta and absent from SDF-1 alpha. SDF-1/PBSF is highly conserved between species, with only one amino acid substitution between the mature human and mouse proteins. SDF-1/PBSF acts via the chemokine receptor CXCR4 and has been shown to be a chemoattractant for T-lymphocytes, monocytes, pro- and pre- B cells, but not neutrophils.

Entrez Symbol:

Cxcl12

Uniprot Name:

Chemokine (C-X-C motif) ligand 12 (Stromal cell-derived factor 1)

Uniprot ID:

Q9QZD1

Data PDF:

CSI20133

Source:

E. coli

Molecular Weight:

~7.9 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Purity:

>97% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 50-100 ng/ml.

Endotoxin Level:

Less than 1EU/μg of rRtSDF-1alpha/CXCL12 as determined by LAL method.

Amino Acid Sequence:

KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA RLKSNNRQVC IDPKLKWIQE YLDKALNK

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/mL. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

This lyophilized preparation is stable at 2-4°C, but should be kept desiccated at -20°C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-4°C.