Recombinant Rat GGTase-II, alpha and beta, Active

Molecule:

GGTase2

Synonyms:

Geranylgeranyltransferase type-2, subunits alpha and beta, Rab geranylgeranyltransferase type-2

Species:

Rat

Cat. No.:

CSI11658 pdf (datasheet)

Quantity/Size:

50 μg

Price:

$650.00 BUY

Description:

GGTase-II catalyzes the transfer of geranylgeranyl moiety onto two C-terminal cysteines of Rab proteins. Composed of an alpha and beta heterodimer and requires Rab escort protein for its catalytic activity.

GGTase-II was shown to exhibit higher affinity towards geranylgeranyl pyrophosphate (Kd= 8 nM) than farnesyl pyrophosphate (Kd= 60 nM). Like FTase and GGTase-I, RabGGT functions as a heterodimer. The alpha subunit has 27% identity to that of CaaX prenylases but contains additional domains, while the beta subunit shows 29% identity to that of FTase. The protein substrates of RabGGT have heterogeneous C termini that usually contain two cysteine residues (CXC), both of which are modified by geranylgeranyl groups. Unlike the CaaX prenylases, RabGGT requires specific accessory proteins known as REPs to guide the interaction with its targets. Some farnesyltransferase inhibitors (FTIs) were identified to inhibit RabGGT activity and induce p53 independent apoptosis in C. elegans.

Concentration:

Typically 1-3 mg/ml

Entrez Symbol:

Rabggtb

Uniprot Name:

Geranylgeranyl transferase type-2 subunit alpha
Geranylgeranyl transferase type-2 subunit beta

Uniprot ID:

Q08602 alpha subunit
Q08603 beta subunit

Data PDF:

CSI11658

Source:

E. coli

Molecular Weight:

50 kDa alpha subunit
38 kDa beta subunit

Physical State:

Liquid

Formulation:

20 mM Tris-HCl, 40 mM NaCl, 5 mM DTT, pH 7.2

Purity:

> 95% as determined by SDS-PAGE analysis.

Storage/Stability:

Stable for 1 year at -80°C. Upon initial thaw, prepare working aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.