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Recombinant Rat CXCL1/GRO alpha/KC

Molecule:

CXCL1

Synonyms:

Chemokine (C-X-C motif) ligand 1, GRO1, CINC-1, CXCL1

Species:

Rat

Cat. No.:

CRG502C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$3,000.00 / Special Price: $1,750.00 BUY

Description:

Recombinant Rat CXCL1/GRO alpha/KC is a single, non-glycosylated polypeptide chain containing 72 amino acids.

Background: Rat CXCL1, also known as CINC-1, belongs to the CXC chemokine family. It is encoded by the GRO gene now designated CXCL1. It was originally purified from media conditioned by IL-1β stimulated rat kidney epithelioid cells. CINC-1 is member of the intercrine alpha (chemokine C-X-C) subfamily of chemokines. CXCL1 is secreted by human melanoma cells, and also expressed by macrophages, neutrophils and epithelial cells. The functional receptor for CXCL1 has been identified as CXCR2. CXCL1 has chemotactic activity for neutrophils, and plays a role in inflammation and wound healing. Amino acid sequence of rat CXCL1 is approximately 68 % identical to the human CXCL1.

Data PDF:

CRG502

Source:

E. coli

Molecular Weight:

~7.8 kDa

Formulation:

Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.

Purity:

>97% by SDS-PAGE and HPLC analyses.

Biological Activity:

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using rat neutrophils is in a concentration range of 10-100 ng/ml.

Endotoxin Level:

<1 EU/μg as determined by LAL method.

Amino Acid Sequence:

APVANELRCQ CLQTVAGIHF KNIQSLKVMP PGPHCTQTEV IATLKNGREACLDPEAPMVQ KIVQKMLKGV PK

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

This lyophilized preparation is stable at 2-8°C. Upon receipt, store desiccated at -20°C. After reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C. For long term storage of reconstituted protein, it is recommended that a carrier protein such as 0.1% BSA or HSA be added. This depends on the particular application. Avoid repeated freeze/thaw cycles.