Synonyms:
Somatomedin C, IGF-I, IGFIA, IGF1, IGFI
Description:
Background: The somatomedins, or insulin-like growth factors (IGFs), comprise a family of peptides that play important roles in mammalian growth and development. IGF1 mediates many of the growth-promoting effects of growth hormone (GH; MIM 139250).
Uniprot Name:
Insulin-like growth factor I
Molecular Weight:
~7.7 kDa
Physical State:
Lyophilized
Formulation:
Lyophilized from a 0.2 οΨΒm filtered concentrated solution in PBS, pH 7.4.
Purity:
>97% by RP-HPLC and SDS-PAGE
Biological Activity:
Fully biologically active when compared to standard. The ED50 determined by a cell proliferation assay using serum free human MCF-7 cells is less than 3 ng/ml.
Endotoxin Level:
<1 EU/οΨΒg as determined by LAL method.
Amino Acid Sequence:
GPETLCGAEL VDALQFVCGP RGFYFNKPTG YGSSIRRAPQ TGIVDECCFR SCDLRRLEMY CAPLKPTKSA
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water to the vial to a concentration not less than 100 οΨΒg/ml. After complete solubilization of the protein, it can be further diluted to other aqueous solutions.
Storage/Stability:
The lyophilized protein is stable at 2-8οΨΑC. Upon receipt, store desiccated at -20οΨΑC. After reconstitution, the preparation is stable for up to one week at 2-8οΨΑC. For maximal stability, apportion the reconstituted preparation into working aliquots
