Recombinant Rat IL-1 beta

Molecule:

IL-1 beta

Synonyms:

IL-1b, Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2

Species:

Rat

Cat. No.:

CRI144B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$185.00 BUY

Description:

IL-1β is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1α and IL-1β bind to the same receptor and has similar biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, B-cell maturation and proliferation, mitogenic FGF-like activity and stimulation of the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1β is a secreted cytokine, IL -1α is predominantly a cell-associated cytokine.

Uniprot Name:

Interleukin-1 beta

Uniprot ID:

Q63264

Data PDF:

CRI144

Source:

E. coli

Molecular Weight:

17.4 kDa, 153 aa

Formulation:

Lyophilized from a sterile filtered (0.2 micron) solution containing 10 mM sodium phosphate, pH 7.4

Purity:

≥ 95.0% by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 < 25 ng/ml, determined by the dose-dependant proliferation of mouse D10S cells.

Endotoxin Level:

≤ 1 EU/μg, determined by kinetic LAL analysis.

Amino Acid Sequence:

MVPIRQLHCR LRDEQQKCLV LSDPCELKAL HLNGQNISQQ VVFSMSFVQG ETSNDKIPVA LGLKGLNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKTK VEFESAQFPN WYISTSQAEH RPVFLGNSNG RDIVDFTMEP VSS

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.