Synonyms:
IL-10, Cytokine synthesis inhibitory factor, CSIF
Description:
IL10 is a cytokine produced primarily by monocytes and to a lesser extent by
lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation.
It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory
molecules on macrophages. It also enhances B cell survival, proliferation, and antibody
production. This cytokine can block NF-kappa B activity, and is involved in the regulation
of the JAK-STAT signaling pathway. Knockout studies in mice suggest the function of this
cytokine as an essential immunoregulator in the intestinal tract.
Uniprot Name:
Interleukin-10
Molecular Weight:
18.8 kDa (161 aa) monomer
Formulation:
Lyophilized from a sterile filtered aqueous solution containing 0.1% Trifluoroacetic Acid
Purity:
≥ 95% by reducing and non-reducing SDS-PAGE
Endotoxin Level:
< 1 EU/µg by kinetic LAL analysis.
Amino Acid Sequence:
MSKGHSIRGD NNCTHFPVSQ THMLRELRAA FSQVKTFFQK KDQLDNILLT DSLLQDFKGY LGCQALSEMI KFYLVEVMPQ AENHGPEIKE HLNSLGEKLK TLWIQLRRCH RFLPCENKSK AVEQVKNDFN KLQDKGVYKA MNEFDIFINC IEAYVTLKMK N
Reconstitution:
Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipet the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for reconstitution. A small amount of precipitate may be seen.
Storage/Stability:
Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.
