Recombinant Rat IL-17 AF heterodimer

Synonyms:

Recombinant Rat Interleukin-17 AF Heterodimer, Il17a/Il17f

Species:

Rat

Cat. No.:

CS326C pdf (datasheet)

Quantity/Size:

1 mg

Price:

$4,820.00 / Special Price: $3,720.00 BUY

Description:

Interleukin-17AF (IL-17AF) is a member of the IL-17 family of proteins produced by a subset of T cells, called Th17, following stimulation with IL-23. Since IL-17AF is thought to signal through the IL-17RA receptor, its biological function is similar to that of IL-17A in that it induces the production of a variety of chemokines, in addition to airway neutrophilia. In regard to these functions, IL-17AF has less activity than the IL-17A homodimer but, greater activity than the IL-17F homodimer. Human and rat IL-17AF both show activity on mouse cells. Recombinant rat IL-17AF is a non-glycosylated, disulfidelinked heterodimer.

Entrez Symbol:

Il17a/Il17f

Uniprot ID:

Q61453/Q5BJ95

Data PDF:

CS326

Source:

E. coli

Molecular Weight:

30.2 kDa (IL-17A:134, IL-17F:135, Total: 269 amino acids aa) dimer

Formulation:

Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)

Purity:

≥ 95% by reducing and non-reducing SDS-PAGE

Biological Activity:

This product demonstrates dose-dependent induction of IL-6 production in 3T3 fibroblasts. Detected by ELISA. Typical ED50 <20 ng/ml.

Endotoxin Level:

≤ 1 EU/μg by kinetic LAL analysis

Amino Acid Sequence:

IL-17A subunit: MAVLIPQSSV CPNAEANNFL QNVKVNLKVL NSLSSKASSR RPSDYLNRST SPWTLSRNED PDRYPSVIWE AQCRHQRCVN AEGKLDHHMN SVLIQQEILV LKREPEKCPF TFRVEKMLVG VGCTCVSSIV RHAS IL-17F subunit: MARRNPKVGL SALQKAGNCP PLEDNSVRVD IRIFNQNQGI SVPRDFQNRS SSPWD YNITR DPDRFPSEIA EAQCRHSGCI NAQG

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year.