Recombinant Rat IL-2

Molecule:

IL-2

Synonyms:

Interleukin-2, aldesleukin, T cell growth factor

Species:

Rat

Cat. No.:

CRI146C pdf (datasheet)

Quantity/Size:

1 mg

Price:

$2,720.00 / Special Price: $2,220.00 BUY

Description:

IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 kDa IL2Rα, 75 kDa IL2Rβ, 64 kDa common gamma chain γc/IL2Rγ) that are present on the cell surface in varying preformed complexes.

Entrez Symbol:

Il2

Uniprot Name:

Interleukin-2

Uniprot ID:

P17108

Data PDF:

CRI146

Source:

E. coli

Molecular Weight:

15.6 kDa

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile-filtered aqueous solution containing 0.1% Trifluoroacetic acid

Purity:

>95 % by reducing and non-reducing SDS-PAGE

Biological Activity:

ED50 ≤ 2 ng/ml as determined by a cell proliferation assay using murine CTLL-2

Specific Activity:

≥ 5.0 × 10e5 U/mg

Endotoxin Level:

≤1 EU/µg of protein by kinetic LAL analysis.

Amino Acid Sequence:

MAPTSSPAKE TQQHLEQLLL DLQVLLRGID NYKNLKLPMM LTFKFYLPKQ ATELKHLQCL ENELGALQRV LDLTQSKSFH LEDAGNFISN IRVTVVKLKG SENKFECQFD DEPATVVEFL RRWIAICQSI ISTMTQ

Reconstitution:

Centrifuge vial prior to opening. Add sterile 10 mM HCl to a concentration of 0.1 mg/ml. DO NOT VORTEX. Allow several minutes for complete reconstitution. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Store as supplied at -20°C to -80°C for up to 1 year. Upon reconstitution, prepare working aliquots and store at -20°C to -80°C. It is recommended that a carrier protein such as 0.1% HSA or BSA is added for long term storage. Avoid repeated freeze-thaw cycles.