IL-33

IL-33

Rat

CS501A pdf (datasheet)

2 μg

$190.00 BUY

Interleukin-33 (IL-33), also known as NF-HEV and DVS 27, is a cytokine belonging to the IL-1 superfamily. It is also a proinflammatory protein that may regulate gene transcription and it induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL -33. IL-33 is constitutively expressed in smooth muscle and airway epithelia and it binds to a high-affinity receptor family member ST2. In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues.

Recombinant Rat IL-33 is a single non-glycosylated polypeptide chain containing 156 amino acid residues that shares 59 % aa and 90% sequence identity with human and mouse IL-33. The product does not contain an affinity tag.

CS501

E. coli

17.4 kDa (156 aa) monomer

Lyophilized from sterile-filtered 20 mM Tris, 300 mM NaCl, pH 8.5

> 95 % by SDS-PAGE and HPLC analyses.

ED50 < 0.05 ng/ml, determined by a cell proliferation assay using mouse D10S cells.

≥ 2.0 × 10e6 IU/mg

< 1 EU/µg as determined by LAL method

SIQGTSLLTE SCALSTYNDQ SVSFVLENGC YVINVEDCGK NQEKDKVLLR YYESSFPAQS GDGVDGKKLM VNMSPIKDTD IWLNANDKDY SVELQKGDVS PPDQAFFVLH KKSSDFVSFE CKNLPGTYIG VKDNQLALVE ENDESCNNIM FKLSKM

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Further dilution should be made in appropriate buffered solutions.

This lyophilized preparation is stable at 2-8°C, but should be stored at -20°C to -80°C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C. Avoid repeated freeze/thaw cycles.