Recombinant Rat IL-33

Molecule:

IL-33

Synonyms:

IL-33

Species:

Rat

Cat. No.:

CS501C pdf (datasheet)

Quantity/Size:

1 mg

Price:

$4,680.00 BUY

Description:

Interleukin-33 (IL-33), also known as NF-HEV and DVS 27, is a cytokine belonging to the IL-1 superfamily. It is also a proinflammatory protein that may regulate gene transcription and it induces helper T cells, mast cells, eosinophils and basophils to produce type 2 cytokines. The induction of type 2 cytokines by IL-33 in vivo is believed to induce the severe pathological changes observed in mucosal organs following administration of IL -33. IL-33 is constitutively expressed in smooth muscle and airway epithelia and it binds to a high-affinity receptor family member ST2. In vivo administration of mature IL-33 promotes increased production of IL-5, IL-13, IgE, and IgA, as well as splenomegaly and inflammatory infiltration of mucosal tissues.

Recombinant Rat IL-33 is a single non-glycosylated polypeptide chain containing 156 amino acid residues that shares 59 % aa and 90% sequence identity with human and mouse IL-33. The product does not contain an affinity tag.

Data PDF:

CS501

Source:

E. coli

Molecular Weight:

17.4 kDa (156 aa) monomer

Formulation:

Lyophilized from sterile-filtered 20 mM Tris, 300 mM NaCl, pH 8.5

Purity:

> 95 % by SDS-PAGE and HPLC analyses.

Biological Activity:

ED50 < 0.05 ng/ml, determined by a cell proliferation assay using mouse D10S cells.

Specific Activity:

≥ 2.0 × 10e6 IU/mg

Endotoxin Level:

< 1 EU/µg as determined by LAL method

Amino Acid Sequence:

SIQGTSLLTE SCALSTYNDQ SVSFVLENGC YVINVEDCGK NQEKDKVLLR YYESSFPAQS GDGVDGKKLM VNMSPIKDTD IWLNANDKDY SVELQKGDVS PPDQAFFVLH KKSSDFVSFE CKNLPGTYIG VKDNQLALVE ENDESCNNIM FKLSKM

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Further dilution should be made in appropriate buffered solutions.

Storage/Stability:

This lyophilized preparation is stable at 2-8°C, but should be stored at -20°C to -80°C for long term storage. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -80°C. Avoid repeated freeze/thaw cycles.