Description:
Placenta growth factor (PlGF) is a member of the PDGF/VEGF family of growth factors
that share a conserved pattern of eight cysteines. Alternate splicing results in at least
three human mature PlGF forms containing 131 (PlGF-1), 152 (PlGF-2), and 203 (PlGF
-3) amino acids (aa) respectively. Only PlGF-2 contains a highly basic heparin-binding 21
aa insert at the C-terminus. In rat only one PlGF that is the equivalent of human PlGF-2
has been identified. Rat PlGF shares 60%, 92%, 62% and 59% aa identity with the
appropriate isoform of human, mouse, canine and equine PlGF. PlGF is mainly found as
variably glycosylated, secreted, 55 - 60 kDa disulfide linked homodimers. Mammalian
cells expressing PlGF include villous trophoblasts, decidual cells, erythroblasts,
keratinocytes and some endothelial cells. Circulating PlGF increases during human
pregnancy, reaching a peak in mid-gestation; this increase is attenuated in preeclampsia.
However, deletion of PlGF in the mouse does not affect development or reproduction.
Postnatally, mice lacking PlGF show impaired angiogenesis in response to ischemia.
PlGF binds and signals through VEGF R1/Flt-1, but not VEGF R2/Flk-1/KDR, while
VEGF binds both but signals only through the angiogenic receptor, VEGF R2. PlGF and
VEGF therefore compete for binding to VEGF R1, allowing high PlGF to discourage
VEGF/VEGF R1 binding and promote VEGF/VEGF R2-mediated angiogenesis.
However, PlGF (especially human PlGF-1) and some forms of VEGF can form dimers
that decrease the angiogenic effect of VEGF on VEGF R2. PlGF-2, like VEGF164/165,
shows heparin-dependent binding of neuropilin (Npn)-1 and Npn-2 and can inhibit nerve
growth cone collapse. PlGF induces monocyte activation, migration, and production of
inflammatory cytokines and VEGF. These activities facilitate wound and bone fracture
healing, but also contribute to inflammation in active sickle cell disease and
atherosclerosis. Circulating PlGF often correlates with tumor stage and aggressiveness,
and therapeutic PlGF antibodies are being investigated to inhibit tumor growth and
angiogenesis.
