Synonyms:
VEGF-A, isoform 164, VEGF164 ,VPF, glioma-derived endothelial cell mitogen
Description:
Vascular Endothelial Growth Factor is a potent growth and angiogenic cytokine. It
stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and
vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role
in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the
induction of tumor metastasis and intraocular neovascular syndromes. VEGF signals
through the three receptors; fms-like tyrosine kinase (flt 1), KDR gene product (the
mouse homolog of KDR is the flk-1 gene product) and the flt4 gene product.
Molecular Weight:
38.7 kDa, disulfide-linked homodimeric protein (165 aa monomer)
Physical State:
Lyophilized
Formulation:
Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, 300 mM NaCl, pH 8.8.
Purity:
> 95 % by SDS-PAGE and HPLC analyses
Biological Activity:
ED50 < 5 ng/ml, determined by proliferation of human umbilical vein endothelial cells (HUVEC).
Specific Activity:
> 2.5 × 10e5 IU/mg
Endotoxin Level:
< 1 EU/µg, by LAL analysis
Amino Acid Sequence:
MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR
Storage/Stability:
Store as supplied at -20°C to -80°C
