Recombinant Rhesus Macaque GM-CSF/CSF2

Molecule:

CSF2

Synonyms:

Granulocyte-Macrophage colony stimulating factor, colony stimulating factor 2, GM-CSF

Species:

Rhesus Macaque

Cat. No.:

CRG134B pdf (datasheet)

Quantity/Size:

10 μg

Price:

$275.00 BUY

Description:

GM-CSF was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine or immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes, macrophages, and eosinophils.

Entrez Symbol:

CSF2

Uniprot Name:

Granulocyte-macrophage colony-stimulating factor

Uniprot ID:

Q9GL44

Data PDF:

CRG134

Source:

E. coli

Molecular Weight:

14.4 kDa (127 aa)

Physical State:

Lyophilized

Formulation:

Lyophilized from a sterile filtered PBS, pH 7.4

Purity:

>98% by SDS-PAGE and HPLC analyses.

Biological Activity:

ED50 < 0.1 ng/ml, as calculated by the dose-dependant stimulation of the proliferation of human TF-1 cells

Specific Activity:

> 1.0 ×107 IU/mg.

Endotoxin Level:

< 0.1 ng/µg of protein.

Amino Acid Sequence:

APARSPSPGT QPWEHVNAIQ EARRLLNLSR DTAAEMNKTV EVVSEMFDLQ EPSCLQTRLE LYKQGLQGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFQSFKENL KDFLLVIPFD CWEPVQE

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water or aqueous buffer to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.

Storage/Stability:

Upon receipt, store at -20°C to -80°C for up to 1 year. Upon reconstitution, store at 2-8°C for up to one month. For long term storage reconstitute in working aliquots containing 0.1% BSA and store at -20°C to -80°C. Avoid repeated freeze-thaw cycles.