Recombinant Rhesus Macaque IL-1 beta

Molecule:

IL-1 beta

Synonyms:

Interleukin-1 beta

Species:

Rhesus Macaque

Cat. No.:

CRI269C pdf (datasheet)

Quantity/Size:

1.0 mg

Price:

$3,800.00 BUY

Description:

Rhesus macaque (Macaca mulatta) IL-1 beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta bind to the same receptor and have similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity, and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine. The 17 kDa mature Rhesus macaque IL-1 beta shares 96% aa sequence identity with other rhesus IL-1 beta and 67% -78% with canine, mouse and rat IL1β.

Uniprot Name:

Interleukin-1 beta

Uniprot ID:

P48090

Data PDF:

CRI269

Source:

E. coli

Molecular Weight:

17.3 kDa (153 aa)

Formulation:

Lyophilized from sterile-filtered PBS, pH 7.4.

Purity:

> 98% by SDS-PAGE and HPLC analyses

Biological Activity:

ED50 is typically 3-10 pg/ml, as determined by the dose-dependant stimulation of D10. G4.1 mouse helper T cells.

Specific Activity:

> 1.0 x 10e8 IU/mg

Endotoxin Level:

< 1EU/µg of rRhIL-1 beta as determined by LAL method.

Amino Acid Sequence:

APVRSLHCTL RDAQLKSLVM SGPYELKALH LQGQDLEQQV VFSMSFVQGE ESNDKIPVAL GLKAKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTRGG QDITDFTMQF VSS

Reconstitution:

Centrifuge vial prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Further dilution should be made in appropriate buffered solutions.

Storage/Stability:

Store lyophilized protein at -20°C to -80°C. After reconstitution, the protein is stable for 1 month at 2-8°C. For long term storage, aliquot and freeze at -20°C to -80°C. Avoid repeated freeze-thaw cycles.