Recombinant SARS-CoV-2 (2019-nCoV) NSP16/2'-O-Methyltransferase (His Tag)

Synonyms:

Replicase polyprotein 1ab, ORF1ab polyprotein, 2'-O-Methyltransferase, Non-structural protein 16, nsp16

Species:

SARS-CoV-2

Cat. No.:

CSI99000 pdf (datasheet)

Quantity/Size:

100 µg

Price:

$540.00 BUY

Description:

2'-O-Methyltransferase/nsp16 is one of 15 chains cleaved from Replicase polyprotein 1ab. 2'-O-Methyltransferase mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs. N7-methyl guanosine cap is a prerequisite for binding of nsp16. Therefore plays an essential role in viral mRNAs cap methylation which is essential to evade immune system.Nonstructural protein 16 (NSP16) / viral 2'O-methyltransferase (2'O-MTase) is highly conserved. The conserved 2'O-MTase activity is important for CoV pathogenesis and NSP16 is a conserved universal target for rapid live attenuated vaccine design in an expanding Coronavirus outbreak setting, such as COVID-19. Targeting on the 2'O-methylation pathway on SARS-CoV replication and pathogenesis can be the treatment options for vaccine and anti-viral drugs development which can against SARS-CoV-2,SARS-CoV, MERS-CoV or other RNA and DNA viruses.

Uniprot Name:

Replicase polyprotein 1ab

Uniprot ID:

P0DTD1

Data PDF:

CSI99000

Source:

E. coli

Molecular Weight:

The recombinant SARS-CoV-2 (2019-nCoV) 2'-O-Methyltransferase consists of 299 amino acids with a predicted molecular mass of 33.46 kDa.

Tag:

His

Formulation:

Sterile 10mM Tris 250mM NaCl, 50% Glycerol pH 7.4

Purity:

>85% as determined by SDS-PAGE

Biological Activity:

Testing in progress

Storage/Stability:

Stable for up to 1 year from date of receipt at -20°C to -80°C. It is recommended to store working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.