Recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (His Tag)

Synonyms:

Nucleoprotein, Protein N, Nucleocapsid Protein, NC

Species:

SARS-CoV-2

Cat. No.:

CSI99002 pdf (datasheet)

Quantity/Size:

100 µg

Price:

$540.00 BUY

Description:

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Uniprot Name:

P0DTC9

Uniprot ID:

Nucleoprotein

Source:

E. coli

Molecular Weight:

The recombinant SARS-CoV-2 (2019-nCoV) Nucleocapsid Protein (His tag) consists of 426 amino acids with a predicted molecular mass of 46.61 kDa.

Tag:

His

Formulation:

Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Purity:

>90% as determined by SDS-PAGE

Biological Activity:

Testing in progress

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipette the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Stable for up to 1 year from date of receipt at -20°C to -80°C After reconstitution, store working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.