Recombinant SARS-CoV-2 (2019-nCoV) Spike S1 RBD Protein(mFc Tag)

Synonyms:

Spike glycoprotein, Spike S1 subunit receptor binding domain

Species:

SARS-CoV-2

Cat. No.:

CSI99008A pdf (datasheet)

Quantity/Size:

100 µg

Price:

$300.00 BUY

Description:

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors that bind S1 are ACE2, angiotensinconverting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, Oacetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARSCoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 mediates fusion of the virion and cellular membranes by acting as a class I viral fusion protein. The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.

Uniprot Name:

Spike glycoprotein

Uniprot ID:

P0DTC2

Source:

HEK293 cells

Molecular Weight:

The recombinant SARS-CoV-2 (2019-nCoV) Spike S1 RBD protein (mFc Tag) consists of 457 amino acids with a predicted molecular mass of 51.5 kDa.

Tag:

mFc

Formulation:

Lyophilized from sterile PBS, pH 7.4.
Normally 5% - 8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Purity:

>95% as determined by SDS-PAGE and SEC-HPLC

Biological Activity:

1. In a functional ELISA, immobilized human ACE2 protein (His tag) at 2μg/mL (100μL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1 RBD Protein (mFc Tag) with the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1 RBD Protein (mFc Tag) of 10-30 ng/mL.

2. Loaded Human ACE2 Protein (His tag) on SA Biosensor, can bind SARS-CoV-2/2019- nCoV Spike S1 RBD Protein (mFc Tag with an affinity constant of 0.562 nM as determined by Octet RED System.

Endotoxin Level:

<1.0 EU per μg protein as determined by the LAL method

Reconstitution:

Centrifuge vial prior to opening. Add sterile distilled water to a concentration of 0.1 mg/mL and gently pipette the solution up and down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution.

Storage/Stability:

Stable for up to 1 year from date of receipt at -20°C to -80°C After reconstitution, store working aliquots at -20°C to -80°C. Avoid repeated freeze-thaw cycles.